Clarifying and Imaging <em>Candida albicans</em> Biofilms
نویسندگان
چکیده
منابع مشابه
Combined imaging of bacteria and oxygen in biofilms.
Transparent sensors for microscopic O(2) imaging were developed by spin coating an ultrathin (<1- to 2-microm) layer of a luminescent O(2) indicator onto coverslips. The sensors showed (i) an ideal Stern-Volmer quenching behavior of the luminescence lifetime towards O(2) levels, (ii) homogeneous measuring characteristics over the sensor surface, and (iii) a linear decline of luminescence lifeti...
متن کاملLabel-free in situ Raman and SERS imaging of biofilms
Biofilms are communities of microorganisms embedded in a matrix of extracellular polymeric substances (EPS), which represent the predominant mode of microbial life. EPS (biopolymers such as polysaccharides, proteins, nucleic acids, lipids, and humic-like substances) play a major role in the formation and maintenance of the biofilm structure and in the protection of the embedded microorganisms a...
متن کاملCorrelative Imaging of Structural and Elemental Composition of Bacterial Biofilms
Synchrotron-based phase contrast tomography (holotomography) and scanning hard X-ray fluorescence microscopy (SXFM) are combined to characterize the three-dimensional (3D) structural and corresponding elemental distribution of bacterial biofilms of Pseudomonas aeruginosa. Samples were fixed without contrast agents or microtomal sectioning. Within an intact microbial community single bacteria ar...
متن کاملClarifying Utterances
The paper argues for the importance of utterances (as opposed to \sen-tences in context") as basic units in dialogue. The main case study is clar-iication: a characterization is provided within KOS, a recently developed synthesis of situation semantics and dialogue games, of the clariications that can follow up on an utterance. This involves outlining: (a) a theory of understanding for dialogue...
متن کاملRemote Hyperspectral Imaging of Endolithic Biofilms Using a Robotic Probe
Many scientists on Earth have concentrated their searches for extraterrestrial life on robotic probes sent to nearby planets and moons. These robotic probes are able to analyze conditions on the planets and transmit data back to Earth. One branch of astrobiology is devoted to duplicating the specified conditions of remote planets and moons on Earth, and identifying the life that survives there ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2020
ISSN: 1940-087X
DOI: 10.3791/60718